STIMULATORY FACTORS FOR ENZYMATIC BIOTIN SYNTHESIS FROM DETHIOBIOTIN IN CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI

被引：16

作者：

OHSHIRO, T

YAMAMOTO, M

BUI, BTS

FLORENTIN, D

MARQUET, A

IZUMI, Y

机构：

[1] TOTTORI UNIV, FAC ENGN, DEPT BIOTECHNOL, TOTTORI 680, JAPAN

[2] UNIV PARIS 06, CNRS, URA 493, CHIM ORGAN BIOL LAB, F-75252 PARIS 05, FRANCE

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 05期

关键词：

D O I：

10.1271/bbb.59.943

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of biotin synthesis from dethiobiotin was found in cell-free extracts of an Escherichia coli bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine (AdoMet) had a significant effect, while methionine and cysteine were inert. The activity was linearly stimulated by increasing protein concentration, When the dialyzed cell-free extracts were used for the reaction, NADP(+), NADPH, and FAD among the well-known cofactors tested promoted the activity. Furthermore, in the presence of AdoMet, cysteine was apparently effective for biotin synthetic activity.

引用

页码：943 / 944

页数：2

共 14 条

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