THE GENE ENCODING TOPOISOMERASE-I FROM THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM

被引：29

作者：

TOSH, K ^{[1
]}

KILBEY, B ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

GENE | 1995年 / 163卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

DNA REPLICATION; ANTIMALARIALS; CHROMOSOME MAPPING; NORTHERN ANALYSIS; PCR CLONING;

D O I：

10.1016/0378-1119(95)00376-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Part of the topoisomerase I (TopoI)-encoding gene from Plasmodium falciparum (Pf) was isolated by PCR from cDNA using oligodeoxyribonucleotides modelled on the highly conserved regions of sequence from other species. The entire TopoI gene was obtained by screening a Pf K1 HindIII-EcoRI genomic library in lambda NM1149 with a random-labeled heterologous probe from the Saccharomyces cerevisiae TopoI gene. DNA sequence analysis revealed an open reading frame of 2520 nt encoding a deduced protein of 839 amino acids (aa) with no detectable introns. The Pf TopoI aa sequence has about 40% identity with most eukaryotic TopoI homologues. The gene is located as a single copy on chromosome 5 and Northern analysis identified a transcript of 3.8 kb.

引用

页码：151 / 154

页数：4

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