A MODEL STRUCTURE OF THE MUSCLE PROTEIN COMPLEX 4CA2+-CENTER-DOT-TROPONIN-C-CENTER-DOT-TROPONIN-I DERIVED FROM SMALL-ANGLE SCATTERING DATA - IMPLICATIONS FOR REGULATION

被引：100

作者：

OLAH, GA ^{[1
]}

TREWHELLA, J ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,DIV CHEM SCI & TECHNOL,LOS ALAMOS,NM 87545

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 43期

关键词：

D O I：

10.1021/bi00209a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here a model structure for 4Ca(2+)-troponin C-troponin I derived from small-angle X-ray and neutron scattering data using a Monte Carlo modeling method. In this model, troponin I appears as a spiral structure that wraps around 4Ca(2+) troponin C which adopts an extended dumbbell conformation similar to that observed in the crystal structures of troponin C. The troponin I spiral has the approximate dimensions of an alpha-helix and winds through the hydrophobic ''cups'' in each globular domain of troponin C. The model is consistent with a body of previously published biochemical data on the interactions between troponin C and troponin I, and suggests the molecular mechanism for the Ca2+-sensitive switch that regulates the muscle contraction/relaxation cycle involves a signal transmitted via the central spiral region of troponin I.

引用

页码：12800 / 12806

页数：7

共 44 条

[21]

KOBAYASHI T, 1994, J BIOL CHEM, V269, P5725

[22] THIN FILAMENT PROTEINS AND THIN FILAMENT-LINKED REGULATION OF VERTEBRATE MUSCLE-CONTRACTION [J].

LEAVIS, PC ;

GERGELY, J .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1984, 16 (03) :235-305

[23] CHARACTERIZATION OF ZERO-LENGTH CROSS-LINKS BETWEEN RABBIT SKELETAL-MUSCLE TROPONIN-C AND TROPONIN-I - EVIDENCE FOR DIRECT INTERACTION BETWEEN THE INHIBITORY REGION OF TROPONIN-I AND THE NH2-TERMINAL, REGULATORY DOMAIN OF TROPONIN-C [J].

LESZYK, J ;

GRABAREK, Z ;

GERGELY, J ;

COLLINS, JH .

BIOCHEMISTRY, 1990, 29 (01) :299-304

[24] BOVINE CARDIAC TROPONIN-T - AMINO-ACID-SEQUENCES OF THE 2 ISOFORMS [J].

LESZYK, J ;

DUMASWALA, R ;

POTTER, JD ;

GUSEV, NB ;

VERIN, AD ;

TOBACMAN, LS ;

COLLINS, JH .

BIOCHEMISTRY, 1987, 26 (22) :7035-7042

[25] MODULATION OF CALMODULIN PLASTICITY IN MOLECULAR RECOGNITION ON THE BASIS OF X-RAY STRUCTURES [J].

MEADOR, WE ;

MEANS, AR ;

QUIOCHO, FA .

SCIENCE, 1993, 262 (5140) :1718-1721

[26] TARGET ENZYME RECOGNITION BY CALMODULIN - 2.4-ANGSTROM STRUCTURE OF A CALMODULIN-PEPTIDE COMPLEX [J].

MEADOR, WE ;

MEANS, AR ;

QUIOCHO, FA .

SCIENCE, 1992, 257 (5074) :1251-1255

[27] SMALL-ANGLE SCATTERING - INFORMATION-CONTENT AND ERROR ANALYSIS [J].

MOORE, PB .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1980, 13 (APR) :168-175

[28]

NGAI SM, 1992, J BIOL CHEM, V267, P15715

[29] TROPONIN-I ENCOMPASSES AN EXTENDED TROPONIN-C IN THE CA2+-BOUND COMPLEX - A SMALL-ANGLE X-RAY AND NEUTRON-SCATTERING STUDY [J].

OLAH, GA ;

ROKOP, SE ;

WANG, CLA ;

BLECHNER, SL ;

TREWHELLA, J .

BIOCHEMISTRY, 1994, 33 (27) :8233-8239

[30]

OLAH GA, 1993, J BIOL CHEM, V268, P16241

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