NUCLEIC-ACID AFFINITY-CHROMATOGRAPHY - PREPARATION AND CHARACTERIZATION OF DOUBLE-STRANDED-RNA AGAROSE

被引：37

作者：

LANGLAND, JO ^{[1
]}

PETTIFORD, SM ^{[1
]}

JACOBS, BL ^{[1
]}

机构：

[1] ARIZONA STATE UNIV,DEPT MICROBIOL,MOLEC & CELLULAR BIOL PROGRAM,TEMPE,AZ 85287

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1995年 / 6卷 / 01期

关键词：

D O I：

10.1006/prep.1995.1004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl) poly(rC), was covalently attached to agarose-adipic acid hydrazide, The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described. (C) 1995 Academic Press, Inc.

引用

页码：25 / 32

页数：8

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