EFFECT OF VARIOUS CARBON-SOURCES ON MICROBIAL LIPASES BIOSYNTHESIS

The aim of these investigations was to study the conditions for the production of extracellular lipases from Penicilliumroqueforti S-86, which was isolated from a commercial sample of roqueforti chese type. As carbon sources there have been used the following compounds: 2% glucose, fructose and sucrosel 1% and 2% butterfat and 2% olive oil. Maximal amount of lipases was produced after six days of incubation grown in the medium with 2% of glucose, initial pH of medium 4.0 at 27°C. Cells of Penicilliumroqueforti grown in the presence of bacto-peptone instead of (NH4)2SO4, as nitrogen source, synthesized maximum quantity of lipases after four days of incubation. The effect of temperature, pH, as well as mono, be and three valent cations: Na+, K+, Ca++, Mn++, Mg++ and Fe+++ on lipase activity was followed. © 1990 Kluwer Academic Publishers.