COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF A 2-PYRIDINONE-BASED HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1)-SPECIFIC REVERSE-TRANSCRIPTASE INHIBITOR IN HUMAN PLASMA

被引：7

作者：

WOOLF, EJ

MATUSZEWSKI, BK

机构：

[1] Department of Drug Metabolism, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania

来源：

PHARMACEUTICAL RESEARCH | 1993年 / 10卷 / 01期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); ASSAY; COLUMN SWITCHING; HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 (HIV-1) REVERSE TRANSCRIPTASE INHIBITOR; ACQUIRED IMMUNE DEFICIENCY SYNDROME;

D O I：

10.1023/A:1018968928742

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method for the determination of a 2-pyridinone-based specific HIV-1 reverse transcriptase inhibitor in human plasma is described. Plasma samples are extracted using phenyl solid phase extraction columns. The extract is analyzed via HPLC using a column-switching system to remove interferences from late-eluting endogenous components. Detection is based on UV absorbance at 314 nm. The assay was linear in the concentration range of 10-500 ng/ml, when 1-ml aliquots of plasma were extracted. The mean precision of the assay, expressed as the coefficient of variation, was 3.8%. The assay has been validated and utilized to support human pharmacokinetic studies.

引用

页码：56 / 60

页数：5

共 6 条

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