PROTOPLAST-INDUCED CURING OF BACTERIOCIN PLASMID IN LACTOCOCCUS-LACTIS SUBSP LACTIS 484

被引：3

作者：

GUPTA, RK

BATISH, VK

机构：

[1] NATL DAIRY RES INST,DIV DAIRY MICROBIOL,KARNAL 132001,HARYANA,INDIA

[2] NATL DAIRY RES INST,PLASMID RES LAB,KARNAL 132001,HARYANA,INDIA

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 73卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1992.tb04986.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plasmid-specified traits like lactose metabolism and bacteriocin production could be eliminated from Lactococcus lactis subsp. lactis 484 culture during production and regeneration of protoplasts with lysozyme at the concentration of 300 mug/ml after 3 h treatment. Plasmid-free strains and cured derivatives harbouring only a single plasmid (2 MDa) were also obtained. Loss of high molecular weight (65 MDa) low copy number Lac plasmid occurred more frequently compared with low molecular weight (2 MDa) high copy number plasmid. Treatment of L. lactis subsp. lactis 484 cells with lysozyme at concentrations of 1000 mug/ml could produce a large number of Lac- Bac- variants at a very high frequency (94%). The curing data confirmed the linkage of Lac and Bac phenotypes to

引用

页码：337 / 341

页数：5

共 21 条

[1] PLASMIDS, LOSS OF LACTOSE METABOLISM, AND APPEARANCE OF PARTIAL AND FULL LACTOSE-FERMENTING REVERTANTS IN STREPTOCOCCUS-CREMORIS-B1 [J].