THE PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST THE MYCOTOXIN DERIVATIVE PATULIN HEMIGLUTARATE

被引：20

作者：

MCELROY, LJ

WEISS, CM

机构：

[1] Department of Microbiology, Beaver Campus, Pennsylvania State University, Monaca

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1993年 / 39卷 / 09期

关键词：

PATULIN; MYCOTOXIN; ANTIBODIES;

D O I：

10.1139/m93-128

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mycotoxin patulin is a toxic, carcinogenic, unsaturated lactone produced by a number of molds. Polyclonal antibodies against patulin hemiglutarate were produced. Specific antibodies against patulin alone, however, were not clearly demonstrated. Because of its low molecular weight, patulin required conjugation to bovine serum albumin (BSA) to increase its immunogenicity. Anti-patulin-hemiglutarate-BSA antibody titer and specificity were determined using indirect and indirect competitive ELISA, respectively. Immunoassays would facilitate detection and quantitation of patulin.

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收藏

页码：861 / 863

页数：3

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