REPLACEMENT OF TOXIC MOBILE PHASES COMMONLY USED IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF RETINOIDS

被引：6

作者：

BESLER, K

KNECHT, U

NOLL, GN

机构：

[1] UNIV GIESSEN,INST PHYSIOL,D-35392 GIESSEN,GERMANY

[2] INST POLIKLIN ARBEITS & SOZIAL MED,D-35392 GIESSEN,GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1994年 / 350卷 / 03期

关键词：

D O I：

10.1007/BF00323185

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Vitamin A isomers are usually separated and analysed by high performance liquid chromatography (HPLC) using an eluent mixture which contains organic solvents such as n-hexane or 1,4-dioxane. However, both are toxic, n-hexane causes a degenerating nerve disease, whereas 1,4-dioxane is supposed to be carcinogenic. In order to avoid preventive handling and potential hazards to health these solvents have been replaced by various mobile phases of n-heptane/tert-butylmethylether. The advantages are better baseline separation of the main retinol isomers and discrimination of even several di- and tri-cis-retinol isomers, low solvent consumption and no known risk so far to health.

引用

页码：182 / 184

页数：3