STEM-CELL FACTOR IS ENCODED AT THE SI-LOCUS OF THE MOUSE AND IS THE LIGAND FOR THE C-KIT TYROSINE KINASE RECEPTOR

We have cloned a partial cDNA encoding murine stem cell factor (SCF) and show that the gene is syntenic with the SI locus on mouse chromosome 10. Using retroviral vectors to immortalize fetal liver stromal cell lines from mice harboring lethal mutations at the SI locus ( SI SI, we have shown that SCF genomic sequences are deleted in these lines. Furthermore, two other mutations at SI, SId and SI12H, are associated with deletions or alterations of SCF genomic sequences. In vivo administration of SCF can reverse the macrocytic anemia and locally repair the mast cell deficiency of SI SId mice. We have also provided biological and physical evidence that SCF is a ligand for the c-kit receptor. © 1990.