INCREASED LEVELS OF SUBSTANCE-P AND CALCITONIN-GENE-RELATED PEPTIDE IN RAT ADJUVANT ARTHRITIS - A COMBINED IMMUNOHISTOCHEMICAL AND RADIOIMMUNOASSAY ANALYSIS

被引：84

作者：

AHMED, M ^{[1
]}

BJURHOLM, A ^{[1
]}

SCHULTZBERG, M ^{[1
]}

THEODORSSON, E ^{[1
]}

KREICBERGS, A ^{[1
]}

机构：

[1] NOVUM, CLIN RES CTR, HUDDINGE, SWEDEN

来源：

ARTHRITIS AND RHEUMATISM | 1995年 / 38卷 / 05期

关键词：

D O I：

10.1002/art.1780380519

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To analyze the occurrence of substance P (SP) and calcitonin gene-related peptide (CGRP) in ankle joints and corresponding dorsal root ganglia (L2-L6) of rats with adjuvant arthritis. Methods. Arthritis was induced by inoculation with heat-killed mycobacteria. The morphologic distribution of SP and CGRP was assessed by immunohistochemical analysis. Tissue concentrations of the neuropeptides were determined by radioimmunoassay. Results. Neuronal CGRP-like immunoreactivity was clearly increased in the synovium and the dorsal root ganglia, whereas the increase in SP-positive structures was less pronounced, The tissue concentrations of SP and CGRP were significantly increased both in ankle joints and in dorsal root ganglia. Conclusion. Levels of sensory neuropeptides are increased under conditions of joint inflammation.

引用

收藏

页码：699 / 709

页数：11

相关论文

共 59 条

[1] EXTRACTION AND QUANTITATION OF NEUROPEPTIDES IN BONE BY RADIOIMMUNOASSAY [J].

AHMED, M ;

SRINIVASAN, GR ;

THEODORSSON, E ;

BJURHOLM, A ;

KREICBERGS, A .

REGULATORY PEPTIDES, 1994, 51 (03) :179-188

[2] EXTRACTION OF NEUROPEPTIDES FROM JOINT TISSUE FOR QUANTITATION BY RADIOIMMUNOASSAY - A STUDY IN THE RAT [J].

AHMED, M ;

BJURHOLM, A ;

RAO, GS ;

THEODORSSON, E ;

KREICBERGS, A .

PEPTIDES, 1994, 15 (02) :317-322

[3] THE SYNOVIUM OF TRANSGENIC ARTHRITIC MICE EXPRESSING HUMAN TUMOR-NECROSIS-FACTOR CONTAINS A HIGH-LEVEL OF NERVE GROWTH-FACTOR [J].

ALOE, L ;

PROBERT, L ;

KOLLIAS, G ;

BRACCILAUDIERO, L ;

SPILLANTINI, MG ;

LEVIMONTALCINI, R .

GROWTH FACTORS, 1993, 9 (02) :149-155

[4] NERVE GROWTH-FACTOR IN THE SYNOVIAL-FLUID OF PATIENTS WITH CHRONIC ARTHRITIS [J].

ALOE, L ;

TUVERI, MA ;

CARCASSI, U ;

LEVIMONTALCINI, R .

ARTHRITIS AND RHEUMATISM, 1992, 35 (03) :351-355

[5] STUDIES ON CARRAGEENAN-INDUCED ARTHRITIS IN ADULT-RATS - PRESENCE OF NERVE GROWTH-FACTOR AND ROLE OF SYMPATHETIC INNERVATION [J].

ALOE, L ;

TUVERI, MA ;

LEVIMONTALCINI, R .

RHEUMATOLOGY INTERNATIONAL, 1992, 12 (05) :213-216

[6] TROPHIC FACTORS AND NEURONAL SURVIVAL [J].

BARDE, YA .

NEURON, 1989, 2 (06) :1525-1534

[7] ENHANCEMENT OF PHAGOCYTOSIS - A NEWLY FOUND ACTIVITY OF SUBSTANCE-P RESIDING IN ITS N-TERMINAL TETRAPEPTIDE SEQUENCE [J].

BARSHAVIT, Z ;

GOLDMAN, R ;

STABINSKY, Y ;

GOTTLIEB, P ;

FRIDKIN, M ;

TEICHBERG, VI ;

BLUMBERG, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 94 (04) :1445-1451

[8] FIXATION AND DEMINERALIZATION OF BONE TISSUE FOR IMMUNOHISTOCHEMICAL STAINING OF NEUROPEPTIDES [J].

BJURHOLM, A ;

KREICBERGS, A ;

SCHULTZBERG, M .

CALCIFIED TISSUE INTERNATIONAL, 1989, 45 (04) :227-231

[9] NORADRENERGIC AND PEPTIDERGIC NERVES IN THE SYNOVIAL-MEMBRANE OF THE SPRAGUE-DAWLEY RAT [J].

BJURHOLM, A ;

KREICBERGS, A ;

AHMED, M ;

SCHULTZBERG, M .

ARTHRITIS AND RHEUMATISM, 1990, 33 (06) :859-865

[10] TACHYKININ MULTIPLICITY IN RAT CENTRAL-NERVOUS-SYSTEM AS STUDIED USING ANTISERA RAISED AGAINST SUBSTANCE-P AND NEUROKININ-A [J].

BRODIN, E ;

LINDEFORS, N ;

DALSGAARD, CJ ;

THEODORSSONNORHEIM, E ;

ROSELL, S .

REGULATORY PEPTIDES, 1986, 13 (3-4) :253-272

← 1 2 3 4 5 6 →