HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF FREE AND TOTAL POLYAMINES IN HUMAN-SERUM AS FLUORESCAMINE DERIVATIVES

被引：52

作者：

KAI, M

OGATA, T

HARAGUCHI, K

OHKURA, Y

机构：

[1] KYUSHU UNIV,FAC PHARMACEUT SCI,DEPT ANALYT CHEM,HIGASHI KU,FUKUOKA 812,JAPAN

[2] KYUSHU UNIV,FAC MED,DEPT INTERNAL MED 3,FUKUOKA 812,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 163卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)81458-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive and simple fluorimetric method for the determination of free and total polyamines, spermidine, spermine, putrescine and cadaverine, in human serum by high-performance liquid chromatography is described. The polyamines, obtained after clean-up of deproteinized serum by Cellex P column chromatography, are converted to their fluorescamine derivatives in the presence of nickel ion which inhibits the reaction of interfering amines with fluorescamine, and the derivatives are separated simultaneously by reversed-phase chromatography (LiChrosorb RP-18) with a linear gradient elution. The lower limits of detection are 10 and 5 pmole for spermine and the others in 0.5 ml of serum, respectively. © 1979.

引用

页码：151 / 160

页数：10

共 20 条

[11]

RUSSELL DH, 1975, CLIN CHEM, V21, P860

[12] POLYAMINES .1. ENHANCED S-ADENOSYL-L-METHIONINE DECARBOXYLASE IN RAPID GROWTH SYSTEMS, AND .2. RELATIONSHIPS BETWEEN POLYAMINE CONCENTRATIONS AND RNA ACCUMULATION [J].