MOLECULAR CHARACTERIZATION OF THE POLLEN-SPECIFIC GENOMIC CLONE NTPG303 AND IN-SITU LOCALIZATION OF EXPRESSION

被引：21

作者：

WETERINGS, K ^{[1
]}

REIJNEN, W ^{[1
]}

WIJN, G ^{[1
]}

VANDEHEUVEL, K ^{[1
]}

APPELDOORN, N ^{[1
]}

DEKORT, G ^{[1
]}

VANHERPEN, M ^{[1
]}

SCHRAUWEN, J ^{[1
]}

WULLEMS, G ^{[1
]}

机构：

[1] UNIV NIJMEGEN,FAC SCI,DEPT EXPTL BOT,RES GRP MOLEC PLANT PHYSIOL,6525 ED NIJMEGEN,NETHERLANDS

来源：

SEXUAL PLANT REPRODUCTION | 1995年 / 8卷 / 01期

关键词：

CONFOCAL LASER SCANNING MICROSCOPY; IN SITU HYBRIDIZATION; NICOTIANA TABACUM; POLLEN-SPECIFIC GENE; PROMOTER ANALYSIS;

D O I：

10.1007/BF00228757

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The localization of transcripts of the pollen-specific gene NTP303 has been determined by means of in situ hybridization in combination with confocal laser scanning microscopy. NTP303 transcripts were first detectable at the mid-bi-nucleate stage of pollen development and persisted even after the pollen tube had reached the ovary. The majority of the NTP303 transcripts were localized in the vegetative cell, and were predominantly present in the apex of the pollen tube. To study the mechanism of regulation, the genomic clone NTPg303 was isolated and characterized. NTPg303 belongs to a gene family of at least five members. No introns are present in its coding region. Regions matching the pollen-specific PB-element TGTGGTT (Twell et al. 1991) have been found.

引用

页码：11 / 17

页数：7

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