IMPROVED SHUTTLE VECTORS FOR HALOFERAX-VOLCANII INCLUDING A DUAL-RESISTANCE PLASMID

被引：49

作者：

HOLMES, M ^{[1
]}

PFEIFER, F ^{[1
]}

DYALLSMITH, M ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

来源：

GENE | 1994年 / 146卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

HALOPHILIC ARCHAEA; PLASMID VECTORS; DNA GYRASE; GYR A;

D O I：

10.1016/0378-1119(94)90844-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(Ap(R))-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(Nb-R)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (Mv(R)) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the Nb-R determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known.

引用

页码：117 / 121

页数：5