ION CLUSTER TECHNIQUES IN DRUG-METABOLISM - AN EXAMPLE OF THE USE OF MIXTURES OF THE [C-12] [C-14] ISOTOPIC FORMS OF THE SYNTHETIC PROSTAGLANDIN CLOPROSTENOL (ESTRUMATE) TO FACILITATE METABOLITE IDENTIFICATION

The synthetic prostaglandin analogue cloprostenol has been prepared radiolabelled with 14C. The isotopic abundance of 14C at position C‐15 was greater than 90% of the theoretical maximum. We have utilized the high abundance of the 14C isotope for metabolism studies by preparing mixtures of [12C]:[14C]cloprostenol such that fragments detected by mass spectrometry contained characteristic isotope clusters analogous to those often obtained using stable isotopes. Copyright © 1979 Heyden & Son Ltd