EXTRACTION AND ISOLATION OF MESSENGER-RNA FROM ADULT ARTICULAR-CARTILAGE

被引：58

作者：

ADAMS, ME

HUANG, DQ

YAO, LY

SANDELL, LJ

机构：

[1] UNIV CALGARY,JOINT INJURY RES GRP,CALGARY T2N 1N4,ALBERTA,CANADA

[2] UNIV WASHINGTON,DEPT ORTHOPAED SURG,SEATTLE,WA 98195

[3] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 202卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90211-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 m guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification. © 1992.

引用

页码：89 / 95

页数：7

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