PURIFICATION OF URIDINE PHOSPHORYLASE FROM CRUDE EXTRACTS OF ESCHERICHIA-COLI EMPLOYING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY WITH AN AQUEOUS 2-PHASE SOLVENT SYSTEM

被引：21

作者：

LEE, YW

SHIBUSAWA, Y

CHEN, FT

MYERS, J

SCHOOLER, JM

ITO, Y

机构：

[1] NHLBI,BETHESDA,MD 20892

[2] N CAROLINA CENT UNIV,DEPT CHEM,DURHAM,NC 27707

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 15-16期

关键词：

D O I：

10.1080/10826079208016351

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This is a preliminary report on a novel liquid-liquid partitioning chromatography method for the purification of recombinant protein, uridine phosphorylase (UrdPase). This system utilizes an aqueous two-phase solvent system, consisting of polyethylene glycol and phosphate buffer, with a newly developed high-speed countercurrent centrifuge (X-axis, XLL type). The system is capable of purifying crude recombinant UrdPase in an efficient manner.

引用

页码：2831 / 2841

页数：11

共 18 条

[1]

Albertsson P. A., 1986, PARTITION CELL PARTI

[2] GENERATION OF A DETAILED PHYSICAL AND GENETIC-MAP OF THE ILV-METE-UDP REGION OF THE ESCHERICHIA-COLI CHROMOSOME [J].