CODING SEQUENCE COMPOSITION FLANKING EITHER SIGNAL ELEMENT ALTERS V(D)J RECOMBINATION EFFICIENCY

被引：26

作者：

BOUBNOV, NV

WILLS, ZP

WEAVER, DT

机构：

[1] DANA FARBER CANC INST,DIV TUMOR IMMUNOL,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 06期

关键词：

D O I：

10.1093/nar/23.6.1060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lymphoid V(D)J rearrangement is targeted by recombination signal sequences (RSS) bordering V, D or J exons, We demonstrate that the DNA composition of flanking coding positions, particularly poly(A) or poly(T) stretches at one or both RSS, diminishes V(D)J recombination up to 100-fold, Positionally correct cleavages occur in the inhibited reactions, since the junctions formed show the same frequency of precision as uninhibited reactions, Open/shut cleavage/rejoining is not increased at a normal RSS in substrates containing inhibitory A/T homopolymers versus random sequence at a second RSS, Thus recombinase action at both cleavage sites is severely disrupted by modified coding sequences.

引用

页码：1060 / 1067

页数：8

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