SELECTIVE ISOLATION OF HIGHLY POLYMORPHIC (DC-DA)N . (DG-DT)N MICROSATELLITES BY STRINGENT HYBRIDIZATION

被引：10

作者：

IIZUKA, M ^{[1
]}

MAKINO, R ^{[1
]}

SEKIYA, T ^{[1
]}

HAYASHI, K ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV ONCOGENE,TOKYO 104,JAPAN

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1993年 / 10卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1016/1050-3862(93)90017-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We defined a hybridization condition for isolation of clones carrying long (dC-dA)n . (dG-dT)n microsatellites from a genomic plasmid library. By using (dC-dA)20 oligonucleotide as a probe and hybridizing under stringent conditions, more than two-thirds of the obtained plasmid carried the repeats that were longer than (dC-dA)14 and highly polymorphic.

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页码：2 / 5

页数：4

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