PURIFICATION AND CHARACTERIZATION OF AN ALKALINE SERINE PROTEASE PRODUCED BY A PSYCHROTROPHIC BACILLUS SP

被引：24

作者：

MARGESIN, R ^{[1
]}

PALMA, N ^{[1
]}

KNAUSEDER, F ^{[1
]}

SCHINNER, F ^{[1
]}

机构：

[1] BIOCHEM GESMBH,KUNDL,AUSTRIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1992年 / 24卷 / 02期

关键词：

ALKALINE SERINE PROTEASE; BACILLUS SP; PSYCHROTROPHIC;

D O I：

10.1016/0168-1656(92)90124-R

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An alkaline psychrotrophic Bacillus sp. was isolated from glacier materials. The strain was found to excrete an alkaline serine protease when cultivated at 18-degrees-C and pH 10.0. The purified protease had a molecular mass of approximately 27 kDa and an isoelectric point > 9.5. Optimal activity was exhibited at 60-degrees-C and pH 11.0. The specific azocaseinolytic, activity of the protease was twice as high as the specific activity of Subtilisin Carlsberg.

引用

页码：203 / 206

页数：4

共 8 条

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