PURIFICATION AND CHARACTERIZATION OF AN ALKALINE SERINE PROTEASE PRODUCED BY A PSYCHROTROPHIC BACILLUS SP

被引:24
作者
MARGESIN, R [1 ]
PALMA, N [1 ]
KNAUSEDER, F [1 ]
SCHINNER, F [1 ]
机构
[1] BIOCHEM GESMBH,KUNDL,AUSTRIA
关键词
ALKALINE SERINE PROTEASE; BACILLUS SP; PSYCHROTROPHIC;
D O I
10.1016/0168-1656(92)90124-R
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An alkaline psychrotrophic Bacillus sp. was isolated from glacier materials. The strain was found to excrete an alkaline serine protease when cultivated at 18-degrees-C and pH 10.0. The purified protease had a molecular mass of approximately 27 kDa and an isoelectric point > 9.5. Optimal activity was exhibited at 60-degrees-C and pH 11.0. The specific azocaseinolytic, activity of the protease was twice as high as the specific activity of Subtilisin Carlsberg.
引用
收藏
页码:203 / 206
页数:4
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