PORCINE HIGH-MOLECULAR-WEIGHT KININOGEN - ITS PURIFICATION AND PROPERTIES AS A THIOL PROTEINASE-INHIBITOR AS COMPARED TO HUMAN HIGH-MOLECULAR-WEIGHT KININOGEN

被引：11

作者：

TANI, Y

OHKUBO, I

HIGASHIYAMA, S

KUNIMATSU, M

SASAKI, M

机构：

[1] NAGOYA CITY UNIV, SCH MED, DEPT BIOCHEM, MIZUHO KU, NAGOYA, AICHI 467, JAPAN

[2] NARA WOMENS UNIV, DEPT HOME ECON, MIZUHO KU, NARA 630, JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1987年 / 88卷 / 02期

关键词：

D O I：

10.1016/0305-0491(87)90323-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. High mol. wt kininogen (HMW kininogen) was purified to a homogeneous state from porcine plasma. 2. The protein exhibited a strong inhibitory activity for thiol proteinases such as ficin, papain and calpain I, whereas it did not inhibit serine proteinases, trypsin and chymotrypsin. 3. The mol. wt, isoelectric point, amino acid and carbohydrate compositions, stabilities to temperature and pH, kinetic constants, and immunological properties of the porcine HMW kininogen were determined and compared with those of human HMW kininogen.

引用

页码：429 / 441

页数：13

共 49 条

[21] STUDIES OF A CALCIUM-ACTIVATED NEUTRAL PROTEASE FROM CHICKEN SKELETAL-MUSCLE .1. PURIFICATION AND CHARACTERIZATION [J].