GLUTAMYL-TRANSFER RNA REDUCTASE-ACTIVITY IN BACILLUS-SUBTILIS IS DEPENDENT ON THE HEMA GENE-PRODUCT

被引：16

作者：

SCHRODER, I ^{[1
]}

HEDERSTEDT, L ^{[1
]}

KANNANGARA, CG ^{[1
]}

GOUGH, SP ^{[1
]}

机构：

[1] CARLSBERG LAB,DEPT PHYSIOL,DK-2500 COPENHAGEN,DENMARK

来源：

BIOCHEMICAL JOURNAL | 1992年 / 281卷

关键词：

D O I：

10.1042/bj2810843

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bacillus subtilis hemAXCDBL operon encodes enzymes for the synthesis of 5-aminolaevulinic acid via the C5 pathway (hemA and hemL) and uroporphyrinogen III (hemB, hemC and hemD). B. subtilis HemA protein (molecular mass 50 kDa) was overexpressed in hemA mutants of both Escherichia coli and B. subtilis. A mutant B. subtilis HemA protein with a Cys to Tyr change at position 105 was also overexpressed. Both wild-type and mutant HemA proteins migrated as oligomers (molecular mass greater-than-or-equal-to 230 kDa) on gel-filtration columns. All column fractions containing wild-type HemA protein had glutamyl-tRNA reductase activity. No glutamyl-tRNA reductase activity was found with the mutant HemA protein. It is concluded that the B. subtilis hemA gene product is identical to, or part of, the glutamyl-tRNA reductase of the C5 pathway.

引用

页码：843 / 850

页数：8

共 38 条

[31]

Sambrook J., 1989, MOL CLONING LAB MANU

[32]

SASARMAN A, 1968, J BACTERIOL, V96, P1882

[33] TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS-SUBTILIS BY DEOXYRIBONUCLEATE [J].

SPIZIZEN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1958, 44 (10) :1072-1078

[34] CLONING OF HEMA FROM RHIZOBIUM SP NGR234 AND SYMBIOTIC PHENOTYPE OF A GENE-DIRECTED MUTANT IN DIVERSE LEGUME GENERA [J].

STANLEY, J ;

DOWLING, DN ;

BROUGHTON, WJ .

MOLECULAR & GENERAL GENETICS, 1988, 215 (01) :32-37

[35] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[36] ISOLATION, NUCLEOTIDE-SEQUENCE, AND PRELIMINARY CHARACTERIZATION OF THE ESCHERICHIA-COLI K-12 HEMA GENE [J].

VERKAMP, E ;

CHELM, BK .

JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4728-4735

[37] BIOSYNTHESIS OF DELTA-AMINOLEVULINATE IN GREENING BARLEY LEAVES .4. ISOLATION OF 3 SOLUBLE ENZYMES REQUIRED FOR THE CONVERSION OF GLUTAMATE TO DELTA-AMINOLEVULINATE [J].

WANG, WY ;

GOUGH, SP ;

KANNANGARA, CG .

CARLSBERG RESEARCH COMMUNICATIONS, 1981, 46 (04) :243-257

[38] IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].

YANISCHPERRON, C ;

VIEIRA, J ;

MESSING, J .

GENE, 1985, 33 (01) :103-119

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