EFFECT OF BILE/PANCREATIC SECRETIONS ON ABSORPTION OF RADIOACTIVE OR STABLE ZINC - IN-VIVO AND IN-VITRO STUDIES

被引:16
作者
FINLEY, JW
JOHNSON, PE
REEVES, PG
VANDERPOOL, RA
BRISKEANDERSON, M
机构
[1] Agricultural Research Service, Grand Forks Human Nutrition Research Center, United States Department of Agriculture, Grand Forks, 58202-9034, ND
关键词
ZINC; HOMEOSTASIS; ABSORPTION; RAT; CACO-2; STABLE ISOTOPIC ZN;
D O I
10.1007/BF02785381
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Biliary/pancreatic (B/P) secretions are a major component of endogenous secretions, and endogenously secreted Zn is a primary means of Zn homeostasis. This study examined whether B/P fluid alters the absorption/reabsorption of Zn and, in doing so, whether this contributes to homeostatic control of Zn. Animal experiments utilized rats fed 10 or 300 mu g Zn/kg diet. An open-ended gut perfusion study in which Zn-65-labeled B/P fluid or Zn-67-labeled and digested diet found significantly decreased Zn absorption from B/P fluid. Although Zn absorption from both sources was less in animals fed diets higher in Zn, there was no interaction of treatment and diet. Further studies utilizing cultured human colon carcinoma cells (CACO-2) as in vitro models of gut enterocytes found that the presence of B/P fluid significantly decreased Zn retention and/or transport and resulted in a redistribution of cellular Zn after 1200 min of incubation. These studies show that a substance in B/P fluid can decrease the absorption of Zn and also suggest that dietary Zn and Zn associated with B/P secretions are absorbed from distinct pools. However, the lack of an interactive effect with diet, and the amount of time required to see differences in CACO-2 cells, suggest that differences in absorption are not a major contributor to Zn homeostasis.
引用
收藏
页码:81 / 96
页数:16
相关论文
共 42 条
[11]   EXCRETION OF RADIOACTIVE ZINC ZN-65 IN BILE, PANCREATIC AND DUODENAL SECRETIONS OF THE DOG [J].
BIRNSTINGL, M ;
STONE, B ;
RICHARDS, V .
AMERICAN JOURNAL OF PHYSIOLOGY, 1956, 186 (03) :377-379
[12]   UPTAKE AND ABSORPTION OF ZINC IN PERFUSED RAT JEJUNUM - THE ROLE OF ENDOGENOUS FACTORS IN THE LUMEN [J].
BONEWITZ, RF ;
VONER, C ;
FOULKES, EC .
NUTRITION RESEARCH, 1982, 2 (03) :301-307
[13]   CHARACTERIZATION AND QUANTITATION OF A ZINC-BINDING LIGAND IN HUMAN-MILK [J].
EVANS, GW ;
JOHNSON, PE .
PEDIATRIC RESEARCH, 1980, 14 (07) :876-880
[14]   PROPOSED MECHANISM FOR ZINC ABSORPTION IN RAT [J].
EVANS, GW ;
GRACE, CI ;
VOTAVA, HJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1975, 228 (02) :501-505
[15]   RELATIVE INFLUENCE OF AMOUNT OF DIETARY ZINC AND INFUSION OF PROTEIN INTO THE DUODENUM ON THE AMOUNT OF ZINC IN BILIARY PANCREATIC SECRETIONS [J].
FINLEY, J ;
JOHNSON, PE .
NUTRITION RESEARCH, 1992, 12 (10) :1217-1228
[16]  
FINLEY JW, 1992, FASEB J, V5, pA920
[17]   A MODEL TO PRODUCE PURE ZINC-DEFICIENCY IN RATS AND ITS USE TO DEMONSTRATE THAT DIETARY PHYTATE INCREASES THE EXCRETION OF ENDOGENOUS ZINC [J].
FLANAGAN, PR .
JOURNAL OF NUTRITION, 1984, 114 (03) :493-502
[18]   COMPETITION FOR ZINC AMONG SERUM-ALBUMIN AND AMINO-ACIDS [J].
GIROUX, EL ;
HENKIN, RI .
BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 273 (01) :64-&
[19]   DISPOSITION OF METALS IN RATS - A COMPARATIVE-STUDY OF FECAL, URINARY, AND BILIARY-EXCRETION AND TISSUE DISTRIBUTION OF 18 METALS [J].
GREGUS, Z ;
KLAASSEN, CD .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1986, 85 (01) :24-38
[20]   PANCREATIC SECRETION IN THE RAT [J].
GROSSMAN, MI .
AMERICAN JOURNAL OF PHYSIOLOGY, 1958, 194 (03) :535-539