SELECTION OF THERMOTOLERANT YEASTS FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) OF CELLULOSE TO ETHANOL

被引:66
作者
BALLESTEROS, I [1 ]
BALLESTEROS, M [1 ]
CABANAS, A [1 ]
CARRASCO, J [1 ]
MARTIN, C [1 ]
NEGRO, MJ [1 ]
SAEZ, F [1 ]
SAEZ, R [1 ]
机构
[1] CTR INVEST ENERGET MEDIOMBIENTALES & TECHNOL, INST ENERGIAS RENOVABLES, AV COMPLUTENSE 22, E-28040 MADRID, SPAIN
关键词
CELLULOSE; SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF); THERMOTOLERANT YEASTS; ETHANOL FERMENTATION; ENZYMATIC HYDROLYSIS;
D O I
10.1007/BF02922610
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A total of 27 yeast strains belonging to the groups Candida, Saccharomyces, and Kluyveromyces were screened for their ability to grow and ferment glucose at temperatures ranging 32-45-degrees-C. K. marxianus and K. fragilis were found to be the best ethanol producing organisms at the higher temperature tested and, so, were selected for subsequent simultaneous saccharification and fermentation (SSF) studies. SSF experiments were performed at 42 and 45-degrees-C, utilizing Solkafloc (10%) as cellulose substrate and a cellulase loading of 15 FPU/g substrate. Best results were achieved at 42-degrees-C with K. marxianus L. G. and K. fragilis L. G., both of which produced close to 38 g/L ethanol and 0.5 ethanol yield, in 78 h.
引用
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页码:307 / 315
页数:9
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