BINDING-SITES FOR TACHYKININ PEPTIDES IN THE BRAIN AND STOMACH OF THE DOGFISH, SCYLIORHINUS-CANICULA

被引：12

作者：

VANGIERSBERGEN, PLM

CONLON, JM

BUCK, SH

机构：

[1] MARION MERRELL DOW RES INST, 2110 E GALBRAITH RD, CINCINNATI, OH 45215 USA

[2] CREIGHTON UNIV, DEPT BIOMED SCI, OMAHA, NE 68178 USA

[3] UNIV CINCINNATI, DEPT PHARMACOL & CELL BIOPHYS, CINCINNATI, OH 45267 USA

来源：

PEPTIDES | 1991年 / 12卷 / 05期

基金：

英国医学研究理事会;

关键词：

DOGFISH; SCYLIORHINUS-CANICULUS; TACHYKININS; TACHYKININ RECEPTORS; G-PROTEIN; I-125]-BOLTON-HUNTER SUBSTANCE-P; I-125]-BOLTON-HUNTER SCYLIORHININ-II; STOMACH; BRAIN;

D O I：

10.1016/0196-9781(91)90075-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. One site specifically bound [I-125]-Bolton-Hunter substance P (BH-SP) and the rank potency of tachykinins to compete for BH-SP binding revealed similarities with the rank potency of an NK1 receptor. The pharmacology of the other site, which specifically bound [I-125]-Bolton-Hunter scyliorhinin II (BH-Scy II), did not resemble any of the mammalian tachykinin receptors. The rank potency to inhibit BH-Scy II binding to this second site was: scyliorhinin II almost-equal-to scyliorhinin I > eledoisin almost-equal-to substance P almost-equal-to neurokinin A > phyllomedusin almost-equal-to physalaemin > [Sar9Met(O2)11]substance P. Neurokinin B and senktide did not displace BH-Scy II binding. In addition, nucleotide analogues inhibited BH-SP binding but not BH-Scy II binding. Our binding data suggest the existence of a mammalian-like NK1 receptor and of a nonmammalian tachykinin receptor in the dogfish.

引用

页码：1161 / 1163

页数：3