MOLECULAR ANALYSIS OF THE MALIC ENZYME GENE (MAE2) OF SCHIZOSACCHAROMYCES-POMBE

被引：40

作者：

VILJOEN, M

SUBDEN, RE

KRIZUS, A

VANVUUREN, HJJ

机构：

[1] UNIV STELLENBOSCH, DEPT MICROBIOL, STELLENBOSCH 7600, SOUTH AFRICA

[2] UNIV STELLENBOSCH, INST BIOTECHNOL, STELLENBOSCH 7600, SOUTH AFRICA

[3] UNIV GUELPH, DEPT MOLEC BIOL & GENET, GUELPH N1G 2W1, ON, CANADA

来源：

YEAST | 1994年 / 10卷 / 05期

关键词：

MAE2; MALIC ACID; WINE; SCHIZOSACCHAROMYCES POMBE;

D O I：

10.1002/yea.320100506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence analysis of a 46-kb HindIII fragment containing the malic enzyme gene (mae2) of Schizosaccharomyces pombe, revealed the presence of an open reading frame of 1695 nucleotides, coding for a 565 amino acid polypeptide. The mae2 gene is expressed constitutively and encodes a single mRNA transcript of 2.0 kb. The mae2 gene was mapped on chromosome III by chromoblotting. The coding region and inferred amino acid sequence showed significant homology with 12 malic enzyme genes and proteins from widely different origins. Eight highly homologous regions were found in these malic enzymes, suggesting that they contain functionally conserved amino acid sequences that are indispensable for activity of malic enzymes. Two of these regions have previously been reported to be NAD- and NADP-binding sites.

引用

页码：613 / 624

页数：12

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[21] POINT MUTATIONS UPSTREAM OF THE YEAST ADH2 POLY(A) SITE SIGNIFICANTLY REDUCE THE EFFICIENCY OF 3'-END FORMATION [J].