DETERMINATION OF GLYOXAL, METHYLGLYOXAL, DIACETHYL, AND 2,3-PENTANEDIONE IN FERMENTED FOODS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：50

作者：

YAMAGUCHI, M ^{[1
]}

ISHIDA, J ^{[1
]}

ZHU, XX ^{[1
]}

NAKAMURA, M ^{[1
]}

YOSHITAKE, T ^{[1
]}

机构：

[1] CHEM INSPECT & TESTING INST,CTR CHEM BIOTESTING,HITA CITY,OITA 877,JAPAN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 01期

关键词：

D O I：

10.1080/10826079408013445

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive and rapid high-performance liquid chromatographic method for the determination of glyoxal, methylglyoxal, diacethyl, and 2,3-pentanedione in fermented foods is described. After extraction of the compounds with methanol, the compounds in the extract are converted into the corresponding fluorescent derivatives by reaction with 1,2-diamino-4,5-methylenedioxybenzene, a fluorogenic reagent for a-dicarbonyl compounds. The derivatives are separated on a reversed-phase column (L-column ODS) with isocratic elution using acetonitrile - 0.5M ammonium acetate, and are detected fluorimetrically. The detection limits are 11.6 - 13.8 fmol per 10-mu l injection for all the compounds at a signal to noise ratio of 3.

引用

页码：203 / 211

页数：9

共 14 条

[1] VICINAL DIKETONES IN BEER .1. A MODIFIED EBC METHOD FOR THE DETERMINATION OF DIACETYL AND OTHER VICINAL DIKETONES IN BEER [J].

ALVAREZ, R ;

HAYA, EF ;

DELGADO, MA ;

FERRETE, FJ .

JOURNAL OF THE INSTITUTE OF BREWING, 1989, 95 (01) :21-23

[2] DETERMINATION OF BIACETYL IN BEER BY LIQUID-CHROMATOGRAPHY WITH SENSITIZED PHOSPHORESCENCE DETECTION [J].

BAUMANN, RA ;

GOOIJER, C ;

VELTHORST, NH ;

FREI, RW ;

STRATING, J ;

VERHAGEN, LC ;

VELDHUYZENDOORDUIN, RC .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1986, 25 (1-3) :195-207

[3] MODIFICATION OF PRILL AND HAMMER METHOD FOR DETERMINING DIACETYL [J].

COGAN, TM .

JOURNAL OF DAIRY SCIENCE, 1972, 55 (03) :382-&

[4]

DEGORCEDUMAS R, 1986, IND ALIMENT AGR, V103, P805

[5] 1,2-DIAMINO-4,5-METHYLENEDIOXYBENZENE AS A HIGHLY SENSITIVE FLUOROGENIC REAGENT FOR ALPHA-DICARBONYL COMPOUNDS [J].

HARA, S ;

YAMAGUCHI, M ;

TAKEMORI, Y ;

YOSHITAKE, T ;

NAKAMURA, M .

ANALYTICA CHIMICA ACTA, 1988, 215 (1-2) :267-276

[6]

Harrison G. A. F., 1965, J I BREWING, V71, P336

[7] MONITORING BACTERIAL METABOLITES IN CULTURED BUTTERMILK BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND HEADSPACE GAS-CHROMATOGRAPHY [J].

MARSILI, RT .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1981, 19 (09) :451-456

[8]

MATSUURA H, 1990, BUNSEKI KAGAKU, V39, P405

[9]

Nachkov D., 1982, Lozarstvo i Vinarstvo, V31, P35

[10]

NAKAMURA M, 1987, CHEM PHARM BULL, V35, P687

← 1 2 →