FUSARIUM-GRAMINEARUM-A-3/5 AS A NOVEL HOST FOR HETEROLOGOUS PROTEIN-PRODUCTION

被引：43

作者：

ROYER, JC

MOYER, DL

REIWITCH, SG

MADDEN, MS

JENSEN, EB

BROWN, SH

YONKER, CC

JOHNSTONE, JA

GOLIGHTLY, EJ

YODER, WT

SHUSTER, JR

机构：

[1] Novo Nordisk Biotech Inc, Davis, CA, 95616

来源：

BIO-TECHNOLOGY | 1995年 / 13卷 / 13期

关键词：

D O I：

10.1038/nbt1295-1479

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a novel fungal expression system which utilizes the Quorn(R) myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.

引用

页码：1479 / 1483

页数：5

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