PROPERTIES OF CHOLINESTERASE AND CARBOXYLESTERASE OF NERVOUS-TISSUE IN PERIPLANETA-AMERICANA

被引：13

作者：

BRICK, IL

BRESTKIN, AP

MANDELSHTAM, JE

机构：

[1] Sechenov Institute of Evolutionary Physiology, Biochemistry Academy of Sciences U.S.S.R., 194223 Leningrad

来源：

INSECT BIOCHEMISTRY | 1979年 / 9卷 / 04期

关键词：

carboxylesterase; Cholinesterase; nervous system; organophosphorus inhibitors;

D O I：

10.1016/0020-1790(79)90089-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rates of enzymic hydrolysis of acetylcholine chloride, acetyl-β-methylcholine chloride, propionylcholine iodide, butyrylcholine iodide, acetylthiocholine iodide (AcTCh) and p-nitrophenyl acetate (p-NPhAc) by homogenates of ganglia from the cockroach Periplaneta americana L. have been studied over a wide range of substrate concentrations. Bimolecular rate constants for the interaction between the esterases hydrolysing these substrates and various organophosphorus inhibitors (OPI) have been determined. The carboxylesterase (CarbE) from cockroach ganglia, which splits p-NPhAc differs from a cholinesterase (ChE) from the same source which hydrolyses choline esters and AcTCh in its sensitivity to some OPI. CarbE as compared with ChE was weakly inhibited by some OPI containing a cationic leaving group and more strongly inhibited by some hydrophobic OPI. Relationships between the structure of the active surface of cockroach ganglion esterases and their sensitivity to OPI are discussed. © 1979.

引用

页码：397 / 401

页数：5

共 30 条

[11]

BRICK IL, 1977, CHIM AGRIC, V15, P40

[12]

COOK B. J., 1965, J INSECT PHYSIOL, V11, P237, DOI 10.1016/0022-1910(65)90071-5

[13] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].