Magnetic Biospecific Affinity Adsorbents for Immunoglobulin and Enzyme Isolation

被引：16

作者：

Griffin, Tadhg ^{[1
]}

Mosbach, Klaus ^{[1
]}

Mosbach, Rolf ^{[2
]}

机构：

[1] Univ Lund, Ctr Chem, S-22007 Lund, Sweden

[2] Univ Dusseldorf, Med Inst Umwelthyg, D-4000 Dusseldorf 1, Germany

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1981年 / 6卷 / 04期

关键词：

Magnetic biospecific affinity adsorbents; biospecific affinity adsorbents; magnetic; affinity adsorbents; magnetic biospecific; adsorbents; magnetic biospecific affinity; immunoglobulin; isolation by magnetic affinity adsorbents; enzymes; glucose-6-phosphate dehydrogenase;

D O I：

10.1007/BF02798279

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a "post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2',5'-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.

引用

页码：283 / 292

页数：10

共 16 条

[1] GLUTARALDEHYDE CYANURIC CHLORIDE AND TETRAAZOTIZED O-DIANISISDINE AS COUPLING REAGENTS IN PASSIVE HEMAGGLUTINATION TEST [J].