VIRULENCE DETERMINANTS OF ESCHERICHIA-COLI O6 EXTRAINTESTINAL ISOLATES ANALYZED BY SOUTHERN HYBRIDIZATIONS AND DNA LONG-RANGE MAPPING TECHNIQUES

被引：25

作者：

BLUM, G

OTT, M

CROSS, A

HACKER, J

机构：

[1] UNIV WURZBURG,INST GENET & MIKROBIOL,RONTGENRING 11,W-8700 WURZBURG,GERMANY

[2] WALTER REED ARMY MED CTR,WALTER REED ARMY INST RES,WASHINGTON,DC 20307

来源：

MICROBIAL PATHOGENESIS | 1991年 / 10卷 / 02期

关键词：

ESCHERICHIA-COLI SEROTYPE O6; EXTRAINTESTINAL ISOLATES; VIRULENCE FACTORS; GENE PROBES; DNA LONG RANGE MAPPING; EPIDEMIOLOGY;

D O I：

10.1016/0882-4010(91)90073-J

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A total of 16 Escherichia coli O6 strains isolated from cases of extraintestinal infections were analysed for the genetic presence and phenotypic expression of fimbrial adhesins (P, S/FIC, type I), aerobactin and hemolysin. In addition restriction fragment length polymorphisms (RFLPs) of Xbal-cleaved genomic DNA of seven selected strains, separated by orthogonal field alternation gel electrophoresis (OFAGE) were determined and virulence-associated DNA probes were used for Southern hybridization studies of the Xbal-cleaved genomic DNAs. The virulence characteristics and hybridization patterns obtained differed between the various isolates. In three isolates hemolysin genes and P fimbrial determinants were located on the same Xbal fragments. Furthermore, multiple copies of FIC determinants (foc) could be detected in two strains. Our data show that the new technique of pulse field electrophoresis together with Southern hybridization represents a powerful tool for the genetic analysis of pathogenic bacteria. © 1991.

引用

页码：127 / 136

页数：10

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