A NEW B-CELL LINE SHOWING A COMPLEX TRANSLOCATION-(8,14,18) AND BCL2-REARRANGEMENT

被引：23

作者：

VANOOTEGHEM, RBC

SMIT, EME

BEISHUIZEN, A

LAMBRECHTS, AC

VANDERBLIJPHILIPSEN, M

SMILDE, TJ

HAGEMEIJER, A

机构：

[1] ERASMUS UNIV ROTTERDAM, DEPT CELL BIOL & GENET, 3000 DR ROTTERDAM, NETHERLANDS

[2] ERASMUS UNIV ROTTERDAM, DEPT IMMUNOL, 3000 DR ROTTERDAM, NETHERLANDS

[3] DR DANIEL DEN HOED CANC CTR, 3008 AE ROTTERDAM, NETHERLANDS

[4] DEPT CLIN GENET, VELDHOVEN, NETHERLANDS

[5] GROOTE ZIEKENGASTHUIS, SHERTOGENBOSCH, NETHERLANDS

来源：

CANCER GENETICS AND CYTOGENETICS | 1994年 / 74卷 / 02期

关键词：

D O I：

10.1016/0165-4608(94)90003-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A cell line named ROS-50 (Rotterdam suspension cell line no. 50) has been established from peripheral blood of a 68-year-old male with acute lymphoblastic leukemia (FAB type L3). Among the aberrations, cytogenetic analysis showed the presence of 14q+, 18q-, and two 8q- marker chromosomes. With fluorescence in situ hybridization (FISH) we characterized the chromosomal translocations, t(8;14) and t(14;18), in which the same chromosome 14 is involved. PCR analysis demonstrated the presence of an IGH-BCL2 rearrangement with a breakpoint in the minor cluster region (mcr) confirming the t(14;18) characteristic for follicular lymphoma. Additional studies showed high expression of BCL2 protein, an early B-cell immunophenotype, and an unusual pattern of IGH gene rearrangement.

引用

页码：87 / 94

页数：8

共 31 条

[1] DETECTION OF THE PHILADELPHIA-CHROMOSOME IN INTERPHASE NUCLEI [J].