NUCLEOTIDE-SEQUENCE OF A CDNA FOR THE BETA-2 SUBUNIT ISOFORM OF NA+,K+-ATPASE FROM HUMAN RETINA

被引：10

作者：

HERNANDO, N

MARTINVASALLO, P

GHOSH, S

GHOSH, PK

SWAROOP, A

COCAPRADOS, M

机构：

[1] YALE UNIV, SCH MED, DEPT OPHTHALMOL & VISUAL SCI, NEW HAVEN, CT 06510 USA

[2] YALE UNIV, SCH MED, NEW HAVEN, CT 06510 USA

[3] UNIV MICHIGAN, KELLOGG EYE CTR, DEPT OPHTHALMOL, ANN ARBOR, MI 48105 USA

[4] UNIV MICHIGAN, KELLOGG EYE CTR, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1189卷 / 01期

关键词：

RETINA CDNA LIBRARY; CDNA LIBRARY; CHARON BS VECTOR; ATPASE; NA+/K+-; BETA2 SUBUNIT ISOZYME;

D O I：

10.1016/0005-2736(94)90287-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using as probe the entire human liver cDNA clone coding for the the beta 2 subunit isoform of the Na+,K+-ATPase, which lacks the initiation codon ATG, and the entire 5'-untranslated region (Martin-Vasallo, P., Dackowski, W., Emanuel, J.R. and Levenson, R. (1989) J. Biol. Chem. 264, 4613-4618), we isolated a larger clone from a directional human adult retina cDNA library (Swaroop, A. and Xu, J. (1993) Cytogenet. Cell Genet. 64, 292-294). This clone, pNH beta 2, shows 100% homology with the nucleotide sequence of the human liver cDNA clone and also contains additional 407 nucleotides in the 5'-untranslated region, the initiation codon and a poly(A) tail. Northern blot hybridization analysis reveals that the human mRNA (3.6 kb) is approx. 300 nucleotides larger than the major transcipt size expressed in rat (3.3 kb). The larger human size mRNA for the human beta 2 Na+,K+-ATPase indicates species differences in gene processing.

引用

页码：109 / 111

页数：3

共 10 条

[1]

Ghosh P K, 1980, Methods Enzymol, V65, P580

[2]

GHOSH S, 1990, J BIOL CHEM, V265, P2935

[3] THE ADHESION MOLECULE ON GLIA (AMOG) IS A HOMOLOG OF THE BETA-SUBUNIT OF THE NA,K-ATPASE [J].