CDNA LIBRARIES FROM HUMAN TISSUES AND CELL-LINES

被引：18

作者：

SWAROOP, A

XU, J

机构：

[1] UNIV MICHIGAN,DEPT OPHTHALMOL,ANN ARBOR,MI 48105

[2] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48105

来源：

CYTOGENETICS AND CELL GENETICS | 1993年 / 64卷 / 3-4期

关键词：

D O I：

10.1159/000133595

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report the construction of 34 cDNA libraries from human tissues and cell lines in lambda phage vectors (Charon BS, lamdbagt11, lambdaSHK, or lamdazapII). The cDNA was synthesized from poly A+ RNA, using oligo-dT as a primer, and size-selected before ligation to vector arms. High-complexity cDNA libraries from human tissues and cell lines should be a valuable resource for genome mapping studies and identification of disease genes.

引用

收藏

页码：292 / 294

页数：3

相关论文

共 21 条

[1] SEQUENCE-ANALYSIS, EXPRESSION AND CHROMOSOMAL LOCALIZATION OF A GENE, ISOLATED FROM A SUBTRACTED HUMAN RETINA CDNA LIBRARY, THAT ENCODES AN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN (IGFBP2) [J].

AGARWAL, N ;

HSIEH, CL ;

SILLS, D ;

SWAROOP, M ;

DESAI, B ;

FRANCKE, U ;

SWAROOP, A .

EXPERIMENTAL EYE RESEARCH, 1991, 52 (05) :549-561

[2]

ANDREWS PW, 1984, LAB INVEST, V50, P147

[3] CHARACTERIZATION OF CDNA FOR THE LARGE SUBUNIT OF THE TRANSCRIPTION INITIATION-FACTOR TFIIF [J].

ASO, T ;

VASAVADA, HA ;

KAWAGUCHI, T ;

GERMINO, FJ ;

GANGULY, S ;

KITAJIMA, S ;

WEISSMAN, SM ;

YASUKOCHI, Y .

NATURE, 1992, 355 (6359) :461-464

[4] PCR-BASED CDNA LIBRARY CONSTRUCTION - GENERAL CDNA LIBRARIES AT THE LEVEL OF A FEW CELLS [J].

BELYAVSKY, A ;

VINOGRADOVA, T ;

RAJEWSKY, K .

NUCLEIC ACIDS RESEARCH, 1989, 17 (08) :2919-2932

[5]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[6] A SIMPLIFIED, ORIENTATION-SPECIFIC CDNA CLONING STRATEGY [J].

DORSSERS, L ;

POSTMES, AMEA .

NUCLEIC ACIDS RESEARCH, 1987, 15 (08) :3629-3629

[7] EXPRESSED SEQUENCE TAGS AND CHROMOSOMAL LOCALIZATION OF CDNA CLONES FROM A SUBTRACTED RETINAL-PIGMENT EPITHELIUM LIBRARY [J].

GIESER, L ;

SWAROOP, A .

GENOMICS, 1992, 13 (03) :873-876

[8] TOWARD A TRANSCRIPTIONAL MAP OF THE HUMAN GENOME [J].

HOCHGESCHWENDER, U .

TRENDS IN GENETICS, 1992, 8 (02) :41-44

[9]

Huynh TV, 1985, DNA CLONING TECHNIQU, V1, P49

[10] HUMAN HOMOLOG OF MURINE TUMOR REJECTION ANTIGEN GP96 - 5'-REGULATORY AND CODING REGIONS AND RELATIONSHIP TO STRESS-INDUCED PROTEINS [J].

MAKI, RG ;

OLD, LJ ;

SRIVASTAVA, PK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5658-5662