THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE

被引：25

作者：

SCHMIDT, M ^{[1
]}

ARNOLD, W ^{[1
]}

NIEMANN, A ^{[1
]}

KLEICKMANN, A ^{[1
]}

PUHLER, A ^{[1
]}

机构：

[1] UNIV BIELEFELD, FAK BIOL, LEHRSTUHL GENET, W-4800 BIELEFELD 1, GERMANY

来源：

GENE | 1992年 / 122卷 / 01期

关键词：

RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; PHAGE-LAMBDA; PMI MUTANT; XANTHOMONAS COMPLEMENTATION; GENE DISRUPTION;

D O I：

10.1016/0378-1119(92)90029-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Interspecific complementation of a Xanthomonas campestris pv. campestris phosphomannose isomerase (PMI) mutant was used to isolate a cosmid from a genomic library of Rhizobium meliloti 2011 carrying the pmi gene of this strain. Subcloning experiments localized the coding region to a 2.0-kb SalI-ClaI fragment. Nucleotide sequence analysis of this fragment indicated the presence of two open reading frames (ORFs), coding for 18- and 43-kDa polypeptides. The analysis of the gene function by gene disruption experiments showed that ORF2 codes for pmi. A comparison of the deduced amino acid sequence with the corresponding sequences of the Pseudomonas aeruginosa and Escherichia coli PMIs revealed no significant homology, indicating that the isolated gene encodes a new type of PMI. The construction of a pmi-deficient mutant of R. meliloti using the sacB-sacR cassette technique showed that the loss of PMI activity does not affect the symbiotic properties of this strain.

引用

页码：35 / 43

页数：9

共 59 条

[51] A BROAD HOST RANGE MOBILIZATION SYSTEM FOR INVIVO GENETIC-ENGINEERING - TRANSPOSON MUTAGENESIS IN GRAM-NEGATIVE BACTERIA [J].

SIMON, R ;

PRIEFER, U ;

PUHLER, A .

BIO-TECHNOLOGY, 1983, 1 (09) :784-791

[52] ROLES OF FLAGELLA, LIPOPOLYSACCHARIDE, AND A CA-2+-DEPENDENT CELL-SURFACE PROTEIN IN ATTACHMENT OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE TO PEA ROOT HAIR TIPS [J].

SMIT, G ;

KIJNE, JW ;

LUGTENBERG, BJJ .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :569-572

[53] CODON PREFERENCE AND ITS USE IN IDENTIFYING PROTEIN CODING REGIONS IN LONG DNA-SEQUENCES [J].

STADEN, R ;

MCLACHLAN, AD .

NUCLEIC ACIDS RESEARCH, 1982, 10 (01) :141-156

[54] THE CURRENT STATUS AND PORTABILITY OF OUR SEQUENCE HANDLING SOFTWARE [J].

STADEN, R .

NUCLEIC ACIDS RESEARCH, 1986, 14 (01) :217-231

[55] BIOSYNTHESIS OF MICROBIAL EXOPOLYSACCHARIDES [J].

SUTHERLAND, IW .

ADVANCES IN MICROBIAL PHYSIOLOGY, 1982, 23 :79-150

[56] IMPROVED ESTIMATION OF SECONDARY STRUCTURE IN RIBONUCLEIC-ACIDS [J].

TINOCO, I ;

BORER, PN ;

DENGLER, B ;

LEVINE, MD ;

UHLENBECK, OC ;

CROTHERS, DM ;

GRALLA, J .

NATURE-NEW BIOLOGY, 1973, 246 (150) :40-41

[57] ON THE EVOLUTION OF TN21-LIKE MULTIRESISTANCE TRANSPOSONS - SEQUENCE-ANALYSIS OF THE GENE (AACC1) FOR GENTAMICIN ACETYLTRANSFERASE-3-I(AAC(3)-I), ANOTHER MEMBER OF THE TN21-BASED EXPRESSION CASSETTE [J].

WOHLLEBEN, W ;

ARNOLD, W ;

BISSONNETTE, L ;

PELLETIER, A ;

TANGUAY, A ;

ROY, PH ;

GAMBOA, GC ;

BARRY, GF ;

AUBERT, E ;

DAVIES, J ;

KAGAN, SA .

MOLECULAR AND GENERAL GENETICS, 1989, 217 (2-3) :202-208

[58] HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI - BACTERIAL MUTATIONS AFFECTING RESTRICTION AND MODIFICATION OF DNA [J].

WOOD, WB .

JOURNAL OF MOLECULAR BIOLOGY, 1966, 16 (01) :118-+

[59] SURFACE CARBOHYDRATES OF RHIZOBIUM .2. LIPOPOLYSACCHARIDES OF RHIZOBIUM [J].

ZEVENHUIZEN, LPTM ;

SCHOLTENKOERSELMAN, I ;

POSTHUMUS, MA .

ARCHIVES OF MICROBIOLOGY, 1980, 125 (1-2) :1-8

← 1 2 3 4 5 6 →