MULTIPLE MESSENGER-RNAS OF MONKEY TYROSINE-HYDROXYLASE

被引:38
作者
ICHIKAWA, S [1 ]
ICHINOSE, H [1 ]
NAGATSU, T [1 ]
机构
[1] NAGOYA UNIV,SCH MED,DEPT BIOCHEM,NAGOYA,AICHI 466,JAPAN
关键词
D O I
10.1016/S0006-291X(05)80933-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The multiplicity of tyrosine hydroxylase (TH) mRNA was examined in two species of primate, Macaca irus and Macaca fuscata, one species of insectivore, Sunkus murinus and one species of rodent, rat. To investigate the TH multiplicity, total RNAs from various species of animals were rerverse transcribed and 5′ portion of TH cDNAs were amplified by polymerase chain reaction. The resulted DNA fragments were analyzed by agarose gel electrophoresis. Two types of TH mRNA corresponding to type 1 and type 2 were detected in adrenal gland and brain of both species of primate. In contrast, only a single form of TH mRNA was detected in Sunkus murinus and rat. © 1990 Academic Press, Inc.
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页码:1331 / 1336
页数:6
相关论文
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