A WHOLE-CELL CANDIDA-ALBICANS ASSAY FOR THE DETECTION OF INHIBITORS TOWARDS FUNGAL CELL-WALL SYNTHESIS AND ASSEMBLY

被引：162

作者：

FROST, DJ

BRANDT, KD

CUGIER, D

GOLDMAN, R

机构：

[1] Anti-Infective Research, Department 47M, Abbott Park, IL 60064-3500, Building AP9A, Abbott Laboratories

来源：

JOURNAL OF ANTIBIOTICS | 1995年 / 48卷 / 04期

关键词：

D O I：

10.7164/antibiotics.48.306

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A whole-cell C. albicans screen was designed to identify novel inhibitors interacting with the synthesis, assembly and regulation of the fungal cell wall. C. albicans was grown in a paired broth assay in 96-well plates with natural product extracts or pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol. Growth was visually examined over a 7-day period and scored into different growth categories. Positives from the sorbitol rescue were then examined under the microscope for morphological alterations and grouped into several morphological classes. Sorbitol protection and cell morphology were indicators of novel antifungal agents from natural product extracts and pure compounds.

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页码：306 / 310

页数：5

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