THE USE OF NEGATIVE-ION THERMOSPRAY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY FOR THE DETERMINATION OF BILE-ACIDS AND THEIR GLYCINE CONJUGATES

A study was carried out on the negative ion thermospray liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry of a group of bile acids and their glycine conjugates. The liquid chromatographic/tandem mass spectrometric experiments were performed using low-energy collisions on a triple-quadrupole mass spectrometer. It was found that relatively high collision energies and collision gas pressures were required to produce fragmentation and that some unusual fragments were produced.