TERT-BUTYLDIPHENYLSILYL DERIVATIZATION FOR LIQUID-CHROMATOGRAPHY AND MASS-SPECTROMETRY

被引：11

作者：

QUILLIAM, MA

YARASKAVITCH, JM

机构：

[1] McMaster Univ, Dep of Chemistry,, Hamilton, Ont, Can, McMaster Univ, Dep of Chemistry, Hamilton, Ont, Can

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1985年 / 8卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

BIOLOGICAL MATERIALS - Chromatographic Analysis - SPECTROSCOPIC ANALYSIS - Applications;

D O I：

10.1080/01483918508067092

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tert-butyldiphenylsilyl (TBDPS) derivatization has been investigated for the purposes of improving the HPLC analysis of polar compounds such as fatty acids, cholesterol and bile acids with ultraviolet absorption and/or mass spectral detection. Very mild reaction conditions (room temperature, 30 min) gave quantitative conversion of carboxyls and sterically accessible hydroxyls.

引用

页码：449 / 461

页数：13

共 15 条

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