A SERUM-FREE MEDIUM FOR THE COURTENAY-MILLS SOFT AGAR ASSAY

被引：4

作者：

WILKS, DP ^{[1
]}

WEST, CML ^{[1
]}

机构：

[1] CHRISTIE HOSP & HOLT RADIUM INST,PATERSON INST CANC RES,MANCHESTER M20 9BX,LANCS,ENGLAND

来源：

INTERNATIONAL JOURNAL OF CELL CLONING | 1991年 / 9卷 / 06期

关键词：

CLONOGENIC ASSAY; SERUM-FREE; CERVIX CARCINOMA; XENOGRAFTS;

D O I：

10.1002/stem.5530090606

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.

引用

页码：559 / 569

页数：11

共 20 条

[1]

CALVO F, 1984, CANCER RES, V44, P4553

[2] GROWTH OF HUMAN TUMOR-CELL COLONIES FROM BIOPSIES USING 2 SOFT-AGAR TECHNIQUES [J].

COURTENAY, VD ;

SELBY, PJ ;

SMITH, IE ;

MILLS, J ;

PECKHAM, MJ .

BRITISH JOURNAL OF CANCER, 1978, 38 (01) :77-81

[3]

DAVIDSON SE, 1991, IN PRESS INT J CANCE

[4] NON-LINEARITY OF COLONY FORMATION BY HUMAN-TUMOR CELLS FROM BIOPSY SAMPLES [J].

ELIASON, JF ;

AAPRO, MS ;

DECREY, D ;

BRINKPETERSEN, M .

BRITISH JOURNAL OF CANCER, 1985, 52 (03) :311-318

[5] CHEMOSENSITIVITY MEASUREMENTS OF HUMAN-TUMOR CELLS BY SOFT AGAR ASSAYS ARE INFLUENCED BY THE CULTURE CONDITIONS [J].

ENDRESEN, L ;

TVEIT, KM ;

RUGSTAD, HE ;

PIHL, A .

BRITISH JOURNAL OF CANCER, 1985, 51 (06) :843-852

[6] TRANSFORMING GROWTH FACTOR-BETA-1 STIMULATES PROLIFERATION OF MORRIS HEPATOMA-CELLS IN SERUM-FREE SOFT AGAR CULTURE SYSTEM SUPPLEMENTED WITH EGF AND INSULIN [J].

GUZIK, K ;

KLEIN, A .

CANCER LETTERS, 1990, 54 (1-2) :51-56

[7] THE HUMAN-TUMOR CLONOGENIC-ASSAY AS A MODEL SYSTEM IN CELL BIOLOGY [J].

HAMBURGER, AW .

INTERNATIONAL JOURNAL OF CELL CLONING, 1987, 5 (02) :89-107

[8] PRIMARY BIOASSAY OF HUMAN TUMOR STEM-CELLS [J].

HAMBURGER, AW ;

SALMON, SE .

SCIENCE, 1977, 197 (4302) :461-463

[9] THE PROPORTION OF STEM-CELLS IN MURINE TUMORS [J].

HILL, RP ;

MILAS, L .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1989, 16 (02) :513-518

[10] HORMONAL-CONTROL OF HUMAN-COLON CARCINOMA CELL-GROWTH IN SERUM-FREE MEDIUM [J].

MURAKAMI, H ;

MASUI, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3464-3468

← 1 2 →