IN-VIVO ASSESSMENT OF MITOCHONDRIAL FUNCTIONALITY IN HUMAN GASTROCNEMIUS-MUSCLE BY P-31 MRS - THE ROLE OF PH IN THE EVALUATION OF PHOSPHOCREATINE AND INORGANIC-PHOSPHATE RECOVERIES FROM EXERCISE

被引：121

作者：

IOTTI, S

LODI, R

FRASSINETI, C

ZANIOL, P

BARBIROLI, B

机构：

[1] UNIV BOLOGNA,IST PATOL MED D CAMPANACCI,CATTEDRA BIOCHIM CLIN,VIA MASSARENTI 9,I-40138 BOLOGNA,ITALY

[2] UNIV MODENA,IST CHIM BIOL,I-41100 MODENA,ITALY

[3] UNIV MODENA,IST RADIOL,I-41100 MODENA,ITALY

来源：

NMR IN BIOMEDICINE | 1993年 / 6卷 / 04期

关键词：

D O I：

10.1002/nbm.1940060404

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study we compared the kinetics of phosphocreatine (PCr) and P(i) recovery, and their dependency on cytosolic pH in 38 normal individuals. Spectra were acquired during rest, work and recovery. A time resolution of 10 s was used to obtain detailed information. The kinetics of PCr and P(i) recovery almost overlapped when the lowest value of cytosolic pH reached during recovery (termed the minimum pH) was < 6.95, while they were completely dissociated when the minimum pH was > 6.95. This result is interpreted as indirect in vivo evidence of the kinetic control exerted by ADP on mitochondrial oxidation. Our results represent a rationale for new experimental conditions to be used in clinical routine studies of pathologies due to primary or secondary mitochondrial malfunction.

引用

页码：248 / 253

页数：6

共 32 条

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