HIGH-FREQUENCY TRANSFORMATION OF STAPHYLOCOCCUS-AUREUS BY ELECTROPORATION

被引：145

作者：

KRAEMER, GR ^{[1
]}

IANDOLO, JJ ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI,DEPT PATHOL,MANHATTAN,KS 66506

来源：

CURRENT MICROBIOLOGY | 1990年 / 21卷 / 06期

关键词：

D O I：

10.1007/BF02199440

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmids carrying resistances for a variety of antibiotics were successfully transformed into Staphylococcus aureus by electroporation. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 × 105 transformants/μg of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (-70°C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity. © 1990 Springer-Verlag New York Inc.

引用

页码：373 / 376

页数：4

共 12 条

[11] HIGH-VOLTAGE ELECTROPORATION OF BACTERIA - GENETIC-TRANSFORMATION OF CAMPYLOBACTER-JEJUNI WITH PLASMID DNA [J].

MILLER, JF ;

DOWER, WJ ;

TOMPKINS, LS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :856-860

[12] A SIMPLE AND RAPID METHOD FOR GENETIC-TRANSFORMATION OF LACTIC STREPTOCOCCI BY ELECTROPORATION [J].

POWELL, IB ;

ACHEN, MG ;

HILLIER, AJ ;

DAVIDSON, BE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (03) :655-660

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