DISTINCT PATTERNS OF PROTECTIVE ANTIBODIES ARE GENERATED AGAINST BORRELIA-BURGDORFERI IN MICE EXPERIMENTALLY INOCULATED WITH HIGH AND LOW-DOSES OF ANTIGEN

被引:58
作者
SCHAIBLE, UE
GERN, L
WALLICH, R
KRAMER, MD
PRESTER, M
SIMON, MM
机构
[1] MAX PLANCK INST IMMUNBIOL,STUBEWEG 51,W-7800 FREIBURG,GERMANY
[2] UNIV NEUCHATEL,INST ZOOL,CH-2000 NEUCHATEL,SWITZERLAND
[3] GERMAN CANC RES CTR,ANGEW IMMUNOL ABT,W-6900 HEIDELBERG 1,GERMANY
[4] UNIV HEIDELBERG,INST IMMUNOL,W-6900 HEIDELBERG,GERMANY
关键词
ANTIBODY RESPONSE; BORRELIA-BURGDORFERI; KINETIC; LYME DISEASE; OSPA; PATHOGENESIS;
D O I
10.1016/0165-2478(93)90056-8
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have studied the development of clinical arthritis and the generation of protective antibodies in two normal, inbred strains of mice either infected by ticks or experimentally (subcutaneous) inoculated with increasing numbers of Borrelia burgdorferi organisms. AKR/N mice developed only mild and DBA/2 mice only marginal clinical arthritis irrespective of the route of infection or the numbers of spirochetes (10-10(8)) inoculated. In contrast, immunodeficient SCID mice developed severe chronic arthritis under similar conditions, but with a delayed onset at lower numbers of needle-inoculated spirochetes or after tick bite. AKR/N and DBA/2 mice inoculated with either 10(4) (and fewer) B. burgdorferi organisms or via experimentally infected ticks generated antibodies with specificities for a variety of B. burgdorferi antigens except those to the outer surface proteins A and B (OspA, OspB). In contrast, mice inoculated with more than 10(4) spirochetes (10(5)-10(8)) developed in addition antibodies to OspA and OspB. Most notably, all three types of immune sera taken from DBA/2 mice showed similar capacities to confer protection on SCID mice against subsequent challenge with viable B. burgdorferi organisms. The data not only demonstrate that the quality of humoral immune responses to B. burgdorferi in mice is determined by the antigenic load, they also indicate the existence of further protective antibodies with specificities distinct from OspA and OspB.
引用
收藏
页码:219 / 226
页数:8
相关论文
共 21 条
[2]   LYME BORRELIOSIS IN SELECTED STRAINS AND AGES OF LABORATORY MICE [J].
BARTHOLD, SW ;
BECK, DS ;
HANSEN, GM ;
TERWILLIGER, GA ;
MOODY, KD .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (01) :133-138
[3]   A SEVERE COMBINED IMMUNODEFICIENCY MUTATION IN THE MOUSE [J].
BOSMA, GC ;
CUSTER, RP ;
BOSMA, MJ .
NATURE, 1983, 301 (5900) :527-530
[4]   ANTIGENS OF BORRELIA-BURGDORFERI RECOGNIZED DURING LYME-DISEASE - APPEARANCE OF A NEW IMMUNOGLOBULIN-M RESPONSE AND EXPANSION OF THE IMMUNOGLOBULIN-G RESPONSE LATE IN THE ILLNESS [J].
CRAFT, JE ;
FISCHER, DK ;
SHIMAMOTO, GT ;
STEERE, AC .
JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (04) :934-939
[5]  
FAHRER H, 1988, SCHWEIZ MED WSCHR, V118, P65
[6]   ROLES OF OSPA, OSPB, AND FLAGELLIN IN PROTECTIVE IMMUNITY TO LYME BORRELIOSIS IN LABORATORY MICE [J].
FIKRIG, E ;
BARTHOLD, SW ;
MARCANTONIO, N ;
DEPONTE, K ;
KANTOR, FS ;
FLAVELL, RA .
INFECTION AND IMMUNITY, 1992, 60 (02) :657-661
[7]   PROTECTION OF MICE AGAINST THE LYME-DISEASE AGENT BY IMMUNIZING WITH RECOMBINANT OSPA [J].
FIKRIG, E ;
BARTHOLD, SW ;
KANTOR, FS ;
FLAVELL, RA .
SCIENCE, 1990, 250 (4980) :553-556
[8]   ELIMINATION OF BORRELIA-BURGDORFERI FROM VECTOR TICKS FEEDING ON OSPA-IMMUNIZED MICE [J].
FIKRIG, E ;
TELFORD, SR ;
BARTHOLD, SW ;
KANTOR, FS ;
SPIELMAN, A ;
FLAVELL, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5418-5421
[9]   MOLECULAR ANALYSIS AND EXPRESSION OF A BORRELIA-BURGDORFERI GENE ENCODING A 22KDA PROTEIN (PC) IN ESCHERICHIA-COLI [J].
FUCHS, R ;
JAURIS, S ;
LOTTSPEICH, F ;
PREACMURSIC, V ;
WILSKE, B ;
SOUTSCHEK, E .
MOLECULAR MICROBIOLOGY, 1992, 6 (04) :503-509
[10]  
GERN L, 1992, IN PRESS J INFECT DI