FUMED-SILICA CONTAINING CARBON-PASTE DEHYDROGENASE BIOSENSORS

被引：46

作者：

WANG, J

LIU, J

机构：

[1] Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 284卷 / 02期

关键词：

BIOSENSORS; ALCOHOL; FUMED SILICA; LACTATE;

D O I：

10.1016/0003-2670(93)85324-D

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The sensitivity and stability of dehydrogenase-based carbon paste biosensors art greatly improved upon adding fumed-silica nanoparticles into the electrode matrix. The enhanced performance is attributed to improved retention of the dehydrogenases and their NAD(+) cofactor on the silica support, and to an electrocatalytic action of fumed silica towards the detection of NADH. Such advantages of the silica modified carbon paste are illustrated with the enzymes alcohol and lactate dehydrogenase, and using batch and flow conditions. The response is characterized with respect to various experimental variables.

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收藏

页码：385 / 391

页数：7

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