RENEWABLE ALCOHOL BIOSENSORS BASED ON ALCOHOL-DEHYDROGENASE NICOTINAMIDE-ADENINE-DINUCLEOTIDE GRAPHITE EPOXY ELECTRODES

被引：44

作者：

WANG, J ^{[1
]}

GONZALEZROMERO, E ^{[1
]}

OZSOZ, M ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,MADRID 34,SPAIN

来源：

ELECTROANALYSIS | 1992年 / 4卷 / 05期

关键词：

ALCOHOLS; BIOSENSORS; GRAPHITE EPOXY;

D O I：

10.1002/elan.1140040505

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD+) have been incorporated within rigid graphite epoxy matrices to yield renewable (by polishing) alcohol sensors. The enzyme and cofactor retain their bioactivity on confinement in the graphite epoxy, with the oxidation current of the NADH formed serving as the response. Because of the intimate contact between the biocatalytic and sensing sites, the reagentless sensor produces steady-state signals within 15-30s. The influence of various experimental variables is explored for optimum analytical performance. Common polishing procedures are used to regenerate fresh bioactive surfaces.

引用

页码：539 / 544

页数：6

共 8 条

[1]

BARMAN TE, 1969, ENZYME HDB, V1, P23

[2]

BICSAC T, 1982, ARCH BIOCH BIOPHYS, V216

[3]

GILBAULT GG, 1976, HDB ENZYMATIC METHOD, P64

[4] CHEMICALLY MODIFIED ELECTRODES FOR THE ELECTROCATALYTIC OXIDATION OF NICOTINAMIDE COENZYMES [J].