NUSG OVEREXPRESSION INHIBITS RHO-DEPENDENT TERMINATION IN ESCHERICHIA-COLI

被引：25

作者：

BUROVA, E ^{[1
]}

GOTTESMAN, ME ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG, INST CANC RES, NEW YORK, NY 10032 USA

来源：

MOLECULAR MICROBIOLOGY | 1995年 / 17卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1995.mmi_17040633.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli NusG protein binds Rho in vitro and is required for efficient Rho-dependent transcription termination in vivo. In this work we examine transcription termination in cells that overexpress NusG. Termination at the Rho-dependent lambda tL1 and lambda tR1 sites was significantly inhibited by excess NusG, whereas the Rho-independent lambda tl site was fully functional. Although Western analysis showed no reduction in the levels of soluble Rho, termination was restored when Rho was also overexpressed. Our data indicate that the ratio of NusG and Rho proteins affects the efficiency of transcription termination.

引用

页码：633 / 641

页数：9

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