AUTOMATED SEQUENTIAL TRACE ENRICHMENT OF DIALYSATES COMBINED WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND AUTOMATED HEART-CUTTING FOR THE DETERMINATION OF THE NUCLEOSIDE 1-(BETA-D-ARABINOFURANOSYL)-5-(1-PROPYNYL)URACIL AND ITS METABOLITE 5-PROPYNYLURACIL IN URINE

被引：11

作者：

COOPER, JDH ^{[1
]}

SHEUNG, CTCF ^{[1
]}

BUICK, AR ^{[1
]}

机构：

[1] WELLCOME RES LABS,DEPT BIOANALYSIS & DRUG METAB,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)E0398-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of a new configuration to control the automated sequential trace enrichment of dialysate (ASTED) system has been used to estimate 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil and its metabolite 5-propynyluracil in urine. The system employs ''heart-cutting'' as a means to improve the efficiency of sample preparation and reduce analysis time. Using this technique the mean within- and between-run imprecision (coefficient of variation) at three different urine analyte concentrations was found to be 1.6 and 3.6% and 1.7 and 3.3% for 5-propynyluracil and 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil, respectively.

引用

页码：15 / 21

页数：7

共 6 条

[1] ONLINE COMBINATION OF DIALYSIS AND COLUMN-SWITCHING LIQUID-CHROMATOGRAPHY AS A FULLY AUTOMATED SAMPLE PREPARATION TECHNIQUE FOR BIOLOGICAL SAMPLES - DETERMINATION OF NITROFURAN RESIDUES IN EDIBLE PRODUCTS
AERTS, MML
BEEK, WMJ
BRINKMAN, UAT
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 500 : 453 - 468
[2] AUTOMATED SEQUENTIAL TRACE ENRICHMENT OF DIALYSATES AND ROBOTICS - A TECHNIQUE FOR THE PREPARATION OF BIOLOGICAL SAMPLES PRIOR TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
COOPER, JDH
TURNELL, DC
GREEN, B
VERILLON, F
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 456 (01): : 53 - 69
[3] AUTOMATIC PREPARATION OF HUMAN-SERUM SAMPLES FOR ANALYSIS OF THE DRUG ENOXIMONE AND ITS SULFOXIDE METABOLITE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
COOPER, JDH
TURNELL, DC
[J]. JOURNAL OF CHROMATOGRAPHY, 1986, 380 (01): : 109 - 116
[4] AUTOMATED PREPARATION OF BIOLOGICAL SAMPLES PRIOR TO HIGH-PRESSURE LIQUID-CHROMATOGRAPHY .2. THE COMBINED USE OF DIALYSIS AND TRACE ENRICHMENT FOR ANALYZING BIOLOGICAL-MATERIAL
COOPER, JDH
TURNELL, DC
[J]. JOURNAL OF AUTOMATIC CHEMISTRY, 1985, 7 (04): : 181 - 184
[5] COOPER JH, 1993, IN PRESS LABORATORY
[6] AUTOMATED SEQUENTIAL PROCESS FOR PREPARING SAMPLES FOR ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
TURNELL, DC
COOPER, JDH
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 395 : 613 - 621

← 1 →