AUTOMATED SEQUENTIAL TRACE ENRICHMENT OF DIALYSATES COMBINED WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND AUTOMATED HEART-CUTTING FOR THE DETERMINATION OF THE NUCLEOSIDE 1-(BETA-D-ARABINOFURANOSYL)-5-(1-PROPYNYL)URACIL AND ITS METABOLITE 5-PROPYNYLURACIL IN URINE

被引：11

作者：

COOPER, JDH ^{[1
]}

SHEUNG, CTCF ^{[1
]}

BUICK, AR ^{[1
]}

机构：

[1] WELLCOME RES LABS,DEPT BIOANALYSIS & DRUG METAB,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)E0398-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of a new configuration to control the automated sequential trace enrichment of dialysate (ASTED) system has been used to estimate 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil and its metabolite 5-propynyluracil in urine. The system employs ''heart-cutting'' as a means to improve the efficiency of sample preparation and reduce analysis time. Using this technique the mean within- and between-run imprecision (coefficient of variation) at three different urine analyte concentrations was found to be 1.6 and 3.6% and 1.7 and 3.3% for 5-propynyluracil and 1-(beta-D-arabinofuranosyl)-5-(1-propynyl)uracil, respectively.

引用

页码：15 / 21

页数：7

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