ESTABLISHING OPTIMAL LYMPHOCYTE GATES FOR IMMUNOPHENOTYPING BY FLOW-CYTOMETRY

被引：234

作者：

LOKEN, MR ^{[1
]}

BROSNAN, JM ^{[1
]}

BACH, BA ^{[1
]}

AULT, KA ^{[1
]}

机构：

[1] MAINE CYTOMETRY RES INST,PORTLAND,ME 04102

来源：

CYTOMETRY | 1990年 / 11卷 / 04期

关键词：

Immunocytometry; immunofluorescence; light scattering; quality control;

D O I：

10.1002/cyto.990110402

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It is possible accurately to distinguish lymphocytes from other leukocyte populations in peripheral blood using the combination of fluorescence associated with CD45/CD14 and forward and orthogonal light scatter. By identifying the cell population of interest based on immunofluorescence, a light scattering window can then be drawn to include all (≥98%) of the lymphocytes. In this manner, maximal recovery of the lymphocytes within a sample can be consistently obtained. The combination of light scattering and immunofluorescence can also be used to define the purity of the gate. The identification of nonlymphocytes within the light scattering gate can then be used to establish an accurate denominator for the percent lymphocytes stained. Once the optimal data acquisition gate has been established and characterized, it is possible to correct subsequent analyses with that particular sample since the reactivity of monoclonal antibodies on monocytes and granulocytes can be accounted for once the nonlymphocytes have been identified as being within the acquisition gate. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：453 / 459

页数：7