MORPHOLOGY OF MAMMALIAN SPERM MEMBRANES DURING DIFFERENTIATION, MATURATION, AND CAPACITATION

被引:70
作者
BEARER, EL [1 ]
FRIEND, DS [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT PATHOL, SAN FRANCISCO, CA 94143 USA
来源
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE | 1990年 / 16卷 / 04期
关键词
Mammalian spermatozoon; Spermatogenesis; Surface membrane;
D O I
10.1002/jemt.1060160403
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mammalian spermatozoon is a highly polarized cell whose surface membrane can be divided into five functionally, structurally, and biochemically distinct domains. These domains are formed during spermatogenesis, continue to be modified during passage through the epididymis, and are further refined in the female reproductive tract. The integrity of these domains appears to be necessary for the sperm to perform its functionfusion with the egg and subsequent fertilization. The domains can be identified morphologically by their surface contours and texture, the content, distribution, and organization of intramembranous particles after freezefracture, and by the density of surface and cytoplasmic electrondense coatings in thin sections. By using a variety of labels that stain carbohydrates (lectins), lipids (filipin and polymyxin B), and monoclonal antibodies to specific membrane constituents, the biochemical composition of these contiguous membrane regions has also been partly elucidated. We review here what is known about the structure, composition, and behavior of each membrane domain in the mature sperm and include some information regarding domain formation during spermatogenesis. The sperm is an excellent model system to study the creation and maintenance of cell polarity, granule exocytosis, and fertilization. Hopefully this review will provide impetus for future studies aimed more directly at addressing the relationship of its morphology to its functions. Copyright1990 WileyLiss, Inc.
引用
收藏
页码:281 / 297
页数:17
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